Protocol Outline for Muscular Dystrophy:-
Mesenchymal Stem Cells (MSCs, CD44+)):- MSCs which differentiate into many types of cells such as bone, cartilage, neuron and so more.
Hematopoietic Stem Cells (HSCs, CD34+)):- HSCs which differentiate into all types of blood cells and also provide enhance environment for MSCs activation.
Endothelial Stem Cells (ESCs, CD31+)):- ESCs which differentiate into all types of blood vessels.
1) To modulate the regeneration process
2) Recruit bone marrow progenitor cells to loci of injury
3) Provides adhesion site for stem cells
4) Platelets fibrin provides migration highway at the site of injury
We also transplant cultured mesenchymal stem cells (isolated from bone marrow and cord tissue) to fulfill the requirement of MSCs cell dose because the cell dose of MSCs is low in bone marrow concentrate.
We transplant “Bone marrow-derived stem cells concentrate (BMSCs)” via intrathecally/intravenously because it provides a rich growth factor (VEGF, TGF-ß, FGF) environment that may enhance the neuron formation. The iliac crest contains bone marrow which is a rich source of the regenerative cells including:-
Muscular Dystrophy Rehabilitation:-
Rehabilitation is the part of stem cell therapy. Muscular Dystrophy patients should have some kind of rehabilitation to help maximize their potential and to help teach techniques which make everyday life easier. Rehabilitation including physiotherapy provides enhanced environment to make stem cell more functional by helping the cell migrating and proliferation fast.
Occupational therapy may help you perform daily activities more easily-especially those involving your hands and arms, such as grooming, dressing, and eating. Assistive devices may be used to help you perform daily tasks.
Physical therapy may improve your ability to perform daily activities and make you feel better.
Follow-up with patients:-
Our medical staff members will be in contact with patients to get complete follow-up at 1, 3, 6 months and I year interval.
Helen et al. 2008 (The New England Journal of Medicine). Cell Therapy for Muscular Dystrophy.
Thomas et al. 2009 (Cell Immunology). Mesenchymal Stem Cells as Anti-inflammatories: Implications for Treatment of Duchenne Muscular Dystrophy.